Application

Anti-S6 Ribosomal Protein, clone 1E9 ZooMAb , Cat. No. ZRB1172, is a highly specific rabbit recombinant monoclonal antibody that targets S6 Ribosomal Protein and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected S6 Ribosomal Protein in human pancreas and mouse pancreas tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected S6 Ribosomal Protein in HeLa, A431, HUVEC, NIH 3T3, Jurkat, MCF-7 cells.Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

His-tagged recombinant fragment corresponding to 136 amino acids from the N-terminal region of human 40S Ribosomal Protein S6.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 1E9 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects 40S ribosomal protein S6. It targets an epitope within the N-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

40S ribosomal protein S6 (UniProt: P62753; also known Phosphoprotein NP33, Small ribosomal subunit protein eS6; RpS6) is encoded by the RPS6 (also known as OK/SW-cl.2) gene (Gene ID: 6194) in human. RpS6 is a highly conserved member of the eukaryotic ribosomal protein eS6 family and is a component of the 40S ribosomal subunit. It plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. It is the major substrate of protein kinases in eukaryote ribosomes and its phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. RpS6 can be phosphorylated on five serine residues (Ser235, 236, 240, 244, and 247) in the region close to the carboxy terminus. Among the serine residues 235 and 236 are shown to be directly phosphorylated by S6Kinase that is subjected to regulation by the nutrient-mTORC1 signaling. Phosphorylation at serine 240/244 is reported to be predominantly nuclear but is also detectable in the cytoplasm, whereas serine 235/236 phosphorylated S6 is almost exclusively localized to the nucleus of primary human cells and is virtually undetectable in the cytoplasm, except in transformed cells where it can also be detected in the cytoplasm. RpS6 is also shown to exist in complex with the Kaposi s sarcoma-associated herpesvirus latency-associated nuclear antigen (LANA). This interaction is mediated by the N-terminal domain of LANA. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Rosner, M., et al. (2011). Amino Acids 40(2); 595-600; Chen, W., and Dittmar, DP (2011). J. Virol. 85(18); 9495-9505; Biever, A., et al. (2015). J. Neurosci. 2015, 35(10); 4113-4130)